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About Dr. Ciro Gargiulo

Personal expertise

Deep clinical understanding of the molecular process underlying auto-immune and immune degenerative pathologies in particular those related to Skeleton and CNS systems with particular attention to those mechanisms associated to metabolic disorders.

•  Since 2005 -2009 working in  Family Medical Practice of HCM city.

• Since 2006 Associate Researcher at Pham Ngoc Thach University of Medicine of HCM city on mesenchymal stem cells project (in vitro phase) with an international co-hosted project with Manchester University and Washington University of Medicine, Faculty of Sport Medicine (ref. : Professor DM Strong).

• Since 2009 to present working as PhD associate researcher at Pham Ngoc Thach University of Medicine on a multiphase stem cell project (vitro, models and phase I, II and III clinical trials humans) in cooperation with the Department of  Medicine /Anatomy and Human Biology of University of Fribourg- Switzerland (Professor Luis Filgueira) and the Faculty of Sport Medicine of Washington University of Medicine Seattle (US). The main project aim is the clinical use of human mesenchymal stem cells (hMSCs) from umbilical cord blood, fat tissue and bone marrow, (autograft and allograft) in treatment of degenerative and auto-immune pathologies associated with metabolic systemic deficiencies.

Published works:

Recent research by Dr. Ciro Gargiulo is published in  3/2013
Adipose tissue can be generated in vitro by using adipocytes from human fat tissue mesenchymal stem cells seeded and cultured on fibrin gel sheet.
Tran CT, Huynh DT, Gargiulo C, Tran le BH, Huynh MH, Nguyen KH, Filgueira L, Strong DM.

Source
Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc, Thach Medical University, Ho Chi Minh City, Vietnam. toaiphd@yahoo.com

Abstract
The current study has developed an innovative procedure to generate ex novo fat tissue by culturing adipocytes from human fat tissue mesenchymal stem cells (hFTMSCs) on fibrin gel sheet towards applications in medicine and cosmetology. Fibrin gel has been obtained by combining two components fibrinogen and thrombin collected by human peripheral blood. By this procedure it was possible to generate blocks of fibrin gel containing adipocytes within the gel that show similar features and consistency to human fat tissue mass. Results were assessed by histological staining methods, fluorescent immune-histochemistry staining as well photos by scanning electron microscopy (SEM) to demonstrate the adhesion and growth of cells in the fibrin gel. This result opens a real possibility for future clinical applications in the treatment of reconstructive and regenerative medicine where the use of stem cell may eventually be a unique solution or in the field of aesthetic medicine where autograft fat stem cells may grant for a safer and better outcome with long lasting results.

IN VITRO CULTURE AND DIFFERENTIATION OF OSTEOBLASTS FROM HUMAN UMBILICAL CORD BLOOD
• Toai, Tran1; Thao, Huynh1; Thao, Nguyen1; Gargiulo, Ciro1; Ngoc, Phan2; Van, Pham3; Strong, D.4
• Cell and Tissue Banking, Volume 11, Number 3, August 2010 , pp. 269-280(12)
• Publisher: Springer

IN VITRO CULTURE OF KERATINOCYTES FROM HUMAN UMBILICAL CORD BLOOD MESENCHYMAL STEM CELLS: THE SAIGONESE CULTURE
• Tran Cong Toai, Huynh Duy Thao, Ciro Gargiulo, Nguyen Phuong Thao, Huynh Minh Tuan, Nguyen Thanh Tung, Luis Filgueira, D Micheal Strong
• Cell and Tissue Banking, 2010, DOI: 10.1007/s10561-010-9174-8
• Publisher: Springer

CULTURE AND DIFFERENTIATION OF OSTEOBLASTS ON CORAL SCAFFOLD FROM HUMAN BONE MARROW MESENCHYMAL STEM CELLS
• Cong Toai Tran, Ciro Gargiulo, Huynh Duy Thao, Huynh Minh Tuan, Luis Filgueira and D. Michael Strong
• Cell and Tissue Banking, 2010, DOI: 10.1007/s10561-010-9208-2
• Publisher: Springer

IN VITRO CULTURE AND DIFFERENTIATION OF OSTEOBLASTS FROM HUMAN UMBILICAL CORD BLOOD AND HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS: A COMPARATIVE STUDY
• Ciro Gargiulo2, Huynh Duy Thao1, Prof. Tran Cong Toai1, Prof. Luis Filgueira2, Prof. Michael Strong3
• Cell Biology Research Progress, 2011, 978-1-61324-669-6: 4
• Publisher: Nova

ADIPOSE TISSUE CAN BE GENERATED IN VITRO BY USING ADIPOCYTES FROM HUMAN FAT TISSUE MESENCHYMAL STEM CELLS SEEDED AND CULTURED ON FIBRIN GEL SHEET
• Tran Cong Toai, Huynh Duy Thao, Ciro Gargiulo, Tran Le Bao Ha, Huynh Minh Hang — Nguyen Khanh Hoa, Luis Filgueira and D. Micheal Strong
• Publisher: Cell and Tissue Bank

ADIPOCYTES AND OSTEOBLASTS FROM HUMAN ADIPOSE FAT TISSUE MESENCHYMAL STEM CELLS CAN BE AN EXCEPTIONAL TOOL IN THE PRODUCTION OF COMPATIBLE AND SAFE BIOMATERIAL ESSENTIAL IN THE FIELD OF RECONSTRUCTIVE AND REGENERATIVE MEDICINE.
• Tran Cong Toai, Huynh Duy Thao, Ciro Gargiulo, Tran Le Bao Ha, Huynh Minh Hang — Nguyen Khanh Hoa, Luis Filgueira and D. Micheal Strong
• Publisher: Springer (chapter book-editing)

AUTOGRAFT TRANSPLANTATION OF OSTEOBLASTS FROM BONE MARROW MESENCHYMAL STEM CELLS SEEDED ON SEA CORAL SCAFFOLD FOR BONE RECONSTRUCTION: A PRELIMINARY STUDY ON RABBITS
• Tran Cong Toai, Huynh Duy Thao, Ciro Gargiulo, Tran Le Bao Ha, Huynh Minh Hang Nguyen Khanh Hoa, Luis Filgueira and D. Micheal Strong
• Publisher: Springer (editing)

OSTEOBLASTS FROM HUMAN BONE MARROW MESENCHYMAL STEM CELLS ON SEA CORAL A RENEWABLE, BIOCOMPATIBLE BONE REPLACEMENT: RESULTS FROM A PHASE III TRIAL
• Tran Cong Toai, Huynh Duy Thao, Ciro Gargiulo, Tran Le Bao Ha, Huynh Minh Hang Nguyen Khanh Hoa, Luis Filgueira and D. Micheal Strong
• Publisher: Springer (editing)

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